We narrowed to 10,212 results for: FIE

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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
JH401: pMVP/Ad/RGD-PK-DEST

Plasmid

#121846

Purpose

pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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SOX D221A/E244A/K246A pACEmam1

Plasmid

#192050

Purpose

Expresses a 3X catalytic mutant of SOX (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (3X catalytic mutant)

Use

Unspecified

Mutation

3X catalytic mutant D221A/E244A/K246A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225063

Purpose

Expression of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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V246 pCEP 4HA B56beta

Plasmid

#14533

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5B B56 beta regulatory subunit of PP2A (PPP2R5B Human)

Tags

4 HA

Expression

Mammalian

Mutation

I346V relative to GenBank ID L42374

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

K466R

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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iMAP-61

Plasmid

#187460

Purpose

gRNA array of iMAP-61

Depositor

Tian Chi

Article

Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

Insert

sgRNA array

Use

Piggybac

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAP118

Plasmid

#105495

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

HupA-SmBiT/HupA-LgBiT

Use

Atc-dependent expression in c. difficile

Promoter

Ptet

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pJL257

Plasmid

#243696

Purpose

A piggyBac vector containing the NDUFB9 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of NDUFB9 (NDUFB9 Human)

Expression

Mammalian

Promoter

endogenous promoter

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-hNHERF2

Plasmid

#47801

Purpose

Expresses human NHERF2 in mammalian cells

Depositor

Randy Hall

Article

Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

Insert

NHERF2 (NHERF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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psgRNA-GST-EGFP-GPIDAF

Plasmid

#213719

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Promoter

CMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-hCYP26A1P-E4-mCherry

Plasmid

#135478

Purpose

Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

mCherry Open reading Frame

Use

Unspecified

Promoter

Short from promoter of human CYP26A1 (312 bp)

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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