We narrowed to 7,729 results for: ALP

1. pIG-837_NY-ESO-1_TCR_tFAS

   Plasmid

   #207500

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tFAS, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

   Plasmid

   #206093

   Purpose

   Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

   Plasmid

   #206098

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV/B-actin

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Brevican scFv [N294A/6]

   Plasmid

   #206772

   Purpose

   Mammalian Expression of Brevican scFV. Derived from hybridoma N294A/6 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Brevican (Rattus norvegicus) recombinant scFV (Bcan Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-FAS-COMP5AP-AviTag-9xHis

   Plasmid

   #157106

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   FAS (FAS Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-IL1R1-COMP5AP-AviTag-9xHis

   Plasmid

   #157320

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   IL1R1 (IL1R1 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pD649-HAsp-FASLG-COMP5AP-AviTag-9xHis

   Plasmid

   #157143

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   FASLG (FASLG Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD649-HAsp-CD8A-COMP5AP-AviTag-9xHis

   Plasmid

   #157118

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CD8A (CD8A Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pD649-HAsp-VSIR-COMP5AP-AviTag-9xHis

   Plasmid

   #157120

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   VSIR (C10orf54 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD649-HAsp-FCAR-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156619

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FCAR (FCAR Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD649-HAsp-ULBP2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156596

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ULBP2 (ULBP2 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-Brevican [N294A/6]

    Plasmid

    #190309

    Purpose

    Mammalian Expression Plasmid of anti-Brevican (Rat). Derived from hybridoma N294A/6.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Brevican (Rattus norvegicus) recombinant Mouse monoclonal antibody (Bcan Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-CD8A-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156553

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD8A (CD8A Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIG-720 CTLA4-GFP R75W

    Plasmid

    #186125

    Purpose

    CTLA4 gene knockin with mutation

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Mutation

    R75W

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTR-337 pUC19-tNGFR-P2A-CTLA4 N

    Plasmid

    #186054

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307_CR-PDHA2S291D

    Plasmid

    #115200

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEX307_CR-PDHA2S293D

    Plasmid

    #115201

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLEX307_CR-PDHA2S291D/S293D

    Plasmid

    #115202

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179433

    Purpose

    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    coreHS4-TRE-cit-coreHS4-mch (DC)
    

    Expression

    Mammalian

    Promoter

    pEF alpha, pRSV

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179426

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Expression

    Mammalian

    Promoter

    pEF alpha, pRSV

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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