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nSYB-GAL80-DD

Plasmid

#124778

Purpose

Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GAL80 (GAL80-DD)

Tags

DD

Expression

Insect

Promoter

nSynaptobrevin

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMF1754

Plasmid

#125585

Purpose

Expresses peroxisomal KillerRed (po-KR) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

po-KillerRed

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PAX6a

Plasmid

#222576

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PAX6a

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PAX6a (PAX6 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT

Plasmid

#19291

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125 Ebo…

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFPGW-UAS-Citrine

Plasmid

#125232

Purpose

For generating UAS-Citrine lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

UAS-Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

UAS

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC.HA Vav3

Plasmid

#14555

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav3 (Vav3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEQ1180

Plasmid

#83805

Purpose

express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

Insert

TRS1

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
lenti-CHMP4B gRNA2

Plasmid

#211629

Purpose

sgRNA-2 against CHMP4B

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA CHMP4B

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-CHMP4B gRNA1

Plasmid

#211628

Purpose

sgRNA-1 against CHMP4B

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA CHMP4B

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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FRP2372

Plasmid

#127578

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA WT HA

Plasmid

#14570

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA (PIK3CA )

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pBp-Control Sponge (CXCR4 sponge)

Plasmid

#22744

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only
pZZMP122

Plasmid

#231756

Purpose

Mammalian expression of SPO11-YF

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-YF (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Y138F

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0248

Plasmid

#162313

Purpose

Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-sfGFP-HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0134

Plasmid

#162307

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame1 (NNT-TCG)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSKB2-TRex

Plasmid

#31349

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor

Tags

His

Expression

Bacterial

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA-POLQ-Halo

Plasmid

#211636

Purpose

sgRNA against POLQ

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA POLQ

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBS mScx (CT#404)

Plasmid

#13958

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (Scx Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
AAV-CK(0.4)GW

Plasmid

#27226

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti_TMv2

Plasmid

#131761

Purpose

frameshift reporter for barcode retrieval

Depositor

Paul Blainey

Article

Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC1 gRNA1

Plasmid

#211630

Purpose

sgRNA-1 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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aCE120

Plasmid

#204491

Purpose

Figure 1. reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
mKate2
EYFP

Use

Synthetic Biology

Tags

none

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.puro_shHuR 3UTR

Plasmid

#110412

Purpose

TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

Depositor

Sandra Martha Gomes Dias

Insert

ELAVL1 (HuR)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-tTA

Plasmid

#19980

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

SYN-tTA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRN104

Plasmid

#127231

Purpose

35S::AFR5::35S terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Mpdelta

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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aCE121

Plasmid

#204492

Purpose

Cadherin circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
Cdh6-mKate2
Cdh1-EYFP

Use

Synthetic Biology

Tags

2A

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0257

Plasmid

#162308

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, HiBiT-stop, frame2 (TTC-G)

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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