Skip to main content
Addgene
Showing: 1481 - 1520 of 11183 results

  9. pTE5418_pMBP-GlcR

    Plasmid
    #221192
    Purpose
    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    glcR (pden4400 from Paracoccus denitrificans)
    Use
    Tags
    10x His tag with E. coli maltose binding protein …
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter and T7 promoter-lacO
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221192-plasmid-map-sequence-id-446698

    Enlarge

  19. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

    Enlarge

  20. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid
    #162654
    Purpose
    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K174, R179, and K182 mutated to alani…
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162654-plasmid-map-sequence-id-320677

    Enlarge

  37. pMCh-1474

    Plasmid
    #82420
    Purpose
    Plasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82420-plasmid-map-sequence-id-156264

    Enlarge

  38. pMCh-1475

    Plasmid
    #82421
    Purpose
    Plasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-1158 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82421-plasmid-map-sequence-id-156262

    Enlarge

  39. pMCh-1476

    Plasmid
    #82422
    Purpose
    Plasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 NED
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1159-1698 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82422-plasmid-map-sequence-id-156258

    Enlarge

  40. pMCh-1477

    Plasmid
    #82423
    Purpose
    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-1158 from nvGW182
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82423-plasmid-map-sequence-id-156255

    Enlarge

Showing: 1481 - 1520 of 11183 results