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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,770 results for: D10

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Showing: 741 - 760 of 3770 results

  3. AAVS1 gRNAs-Cas9D10A-mRuby

    Plasmid
    #252799
    Purpose
    Plasmid for paired targeting of AAVS1 locus via Cas9(D10A)nickase
    Depositor
    Norman Davey
    Insert
    Cas9(D10A)
    Tags
    mRuby
    Expression
    Mammalian
    Available Since
    March 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    252799-plasmid-map-sequence-id-503943

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  5. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

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  6. pPD1024 EF1alpha TetON3G in TU5

    Plasmid
    #139261
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV5
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    hEF1alpha
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139261-plasmid-map-sequence-id-325374

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  7. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid
    #139257
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV5
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139257-plasmid-map-sequence-id-325370

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  15. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  16. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

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  18. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249079-plasmid-map-sequence-id-496312

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Showing: 741 - 760 of 3770 results