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We narrowed to 1,280 results for: MS2

Showing: 741 - 760 of 1280 results

  3. pYPQ-dSpRY-Act3.0

    Plasmid
    #158416
    Purpose
    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    158416-plasmid-map-sequence-id-337116

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  4. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

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  6. pYPQ-SpRY-Act3.0

    Plasmid
    #178957
    Purpose
    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178957-plasmid-map-sequence-id-354306

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  7. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Article
    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131132-plasmid-map-sequence-id-255500

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  8. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  9. pPPC001

    Plasmid
    #171138
    Purpose
    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171138-plasmid-map-sequence-id-344761

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  11. pYPQ-CBE-SpRYn-Act3.0

    Plasmid
    #178958
    Purpose
    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178958-plasmid-map-sequence-id-354326

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  12. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  13. MCP-NMS

    Plasmid
    #210705
    Purpose
    This plasmid express EF1a promoter driven MCP-NMS transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210705-plasmid-map-sequence-id-416249

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  14. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210706-plasmid-map-sequence-id-416266

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  15. MCP-3x9aaTAD

    Plasmid
    #210707
    Purpose
    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210707-plasmid-map-sequence-id-416252

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  16. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

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  17. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  18. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  19. pCK341

    Plasmid
    #192640
    Purpose
    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dSpRY has s…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192640-plasmid-map-sequence-id-381518

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  20. pJZC34

    Plasmid
    #62331
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA
    Promoter
    CMV and U6
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62331-plasmid-map-sequence-id-102253

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Showing: 741 - 760 of 1280 results