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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,002 results for: Streptococcus

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Showing: 741 - 760 of 1002 results

  1. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  6. PV225

    Plasmid
    #132334
    Purpose
    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Available Since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132334-plasmid-map-sequence-id-258129

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  7. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid
    #75400
    Purpose
    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    hCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    35S
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75400-plasmid-map-sequence-id-146044

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  10. pKMW281_dSpRY

    Plasmid
    #244822
    Purpose
    Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)
    Depositor
    Jennifer Doudna
    Article
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Insert
    dSpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CAG
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244822-plasmid-map-sequence-id-486583

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  20. AA246

    Plasmid
    #215946
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215946-plasmid-map-sequence-id-431539

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Showing: 741 - 760 of 1002 results