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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 741 - 760 of 3270 results

  2. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135013-plasmid-map-sequence-id-267601

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  3. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90979-plasmid-map-sequence-id-180505

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  5. p330 VQR

    Plasmid
    #101730
    Purpose
    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101730-plasmid-map-sequence-id-192072

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  6. pLdCH

    Plasmid
    #84291
    Purpose
    Express gRNA and Cas9 in Leishmania with Hygromycin resistance
    Depositor
    Greg Matlashewski
    Publication
    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and Cas9
    Use
    CRISPR; Leishmania
    Promoter
    L. donovani ribosome RNA promoter
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    84291-plasmid-map-sequence-id-170879

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  20. CMV-hCas9-T2A-mStrawberry

    Plasmid
    #187224
    Purpose
    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.
    Depositor
    Jennifer Mitchell
    Publication
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Inserted T2A-mStrawberry at 3' terminal of M…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    187224-plasmid-map-sequence-id-367392

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Showing: 741 - 760 of 3270 results