We narrowed to 7,729 results for: ALP

1. pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

   Plasmid

   #179427

   Purpose

   Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-1.2kb-mch
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVE-FH-Dnd1-IN

   Plasmid

   #70072

   Purpose

   2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_NRAS_p.Y64D

   Plasmid

   #81666

   Purpose

   Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   Y64D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_TNF_p.S157R

   Plasmid

   #81344

   Purpose

   Gateway Donor vector containing TNF , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TNF (TNF Human)
   

   Use

   Gateway entry vector

   Mutation

   S157R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

   Plasmid

   #96888

   Purpose

   expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

   Depositor

   Hening Lin

   Article

   Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

   Insert

   Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
   

   Tags

   FLAG-tagged

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-36, mutated lysine 351 to ar…

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. G13-CASE

   Plasmid

   #168127

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at F125/D126 within GNA13

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223-PDGFRA

   Plasmid

   #23892

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   Gateway donor vector

   Mutation

   M260I

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_FGF1_WT

   Plasmid

   #81825

   Purpose

   Gateway Donor vector containing FGF1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FGF1 (FGF1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid

    #154072

    Purpose

    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Inserts

    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    

    Tags

    GST, His6, and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Gi2-CASE

    Plasmid

    #168121

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at C112/E115 within GNAI2

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223-CSNK2A1

    Plasmid

    #23678

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. MSCV-Flag-ER-Hoxb8-IRES-GFP

    Plasmid

    #222292

    Purpose

    Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    David Sykes, Mark Kamps

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223-CSNK1A1

    Plasmid

    #23355

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pAB2076 pAAV REPAIR.t1

    Plasmid

    #176323

    Purpose

    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    

    Use

    AAV and CRISPR

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    EFS (short EF1alpha) and hU6

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G12-CASE

    Plasmid

    #190714

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.

    Inserts

    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Nluc is inserted at A133/F134 within GNA12 and cp…

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-EIF2AK4

    Plasmid

    #23840

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    Gateway donor vector

    Mutation

    F15S

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only