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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,438 results for: JUN

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Showing: 7581 - 7600 of 18438 results

  1. LSB-hsa-miR-3074-5p

    Plasmid
    #103408
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-3074-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-3074-5p target (MIR3074 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103408-plasmid-map-sequence-id-195865

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  2. LSB-hsa-miR-30a-5p

    Plasmid
    #103410
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-30a-5p target (MIR30A Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103410-plasmid-map-sequence-id-195869

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  3. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138146-plasmid-map-sequence-id-272533

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  4. VWF-pHluorin-mRFP

    Plasmid
    #234367
    Purpose
    Von Willebrand factor fused in tandem to superecliptic pHluorin and inserted into pmRFP1-N1 vector from Addgene (Plasmid No: 54635)/Monitoring of WPB pH, Visualization of WPB exocytosis
    Depositor
    Volker Gerke
    Article
    Terglane et al PLoS One. 2022 Jun 29;17(6):e0270299. do
    Insert
    Von Willebrand factor (VWF Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    234367-plasmid-map-sequence-id-469472

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  8. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  11. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  12. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

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  15. pBeta-lacZ-attBx2

    Plasmid
    #124445
    Purpose
    Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.
    Depositor
    Yingguang Frank Chan
    Article
    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554
    Type
    Empty backbone
    Use
    Enhancer reporter assay construct
    Tags
    LacZ
    Expression
    Mammalian
    Promoter
    beta globin minimal promoter
    Available Since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    124445-plasmid-map-sequence-id-242276

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Showing: 7581 - 7600 of 18438 results