We narrowed to 18,339 results for: Mut

1. pCR8/GW/TOPO-ETV1

   Plasmid

   #98623

   Purpose

   Gateway entry TOPO TA cloning vector for ETV1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ETV1 (Etv1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG CMV14 / BRAF-D594N

   Plasmid

   #131713

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D594N

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEZYeGFP-SARS-CoV-2E GGG

   Plasmid

   #224582

   Purpose

   To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

   Depositor

   Isabel Mérida

   Article

   Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

   Insert

   SARS-CoV-2E GGG (E SARS-CoV-2)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PBM (LLV) mutated to GGG

   Promoter

   CMV

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG CMV14 / BRAF-G469A

   Plasmid

   #131711

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G469A

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bxb1Donor_WT_ADRB2_LucReporter

   Plasmid

   #129788

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LIC1B_Caspase-LOVN7C-7 C450A

   Plasmid

   #104628

   Purpose

   bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

   Depositor

   Jim Wells

   Article

   Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Caspase-3 (CASP3 Human)
   

   Use

   Tags

   His and LOV2

   Expression

   Bacterial

   Mutation

   C450A (dark-locked and light-insensitive mutation)

   Promoter

   T7

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

   Plasmid

   #68345

   Purpose

   A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   puromycin
   hCas9
   

   Use

   CRISPR; Flp/frt

   Tags

   linked to red flouresent protein gene mKateII and…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_ATP10B

   Plasmid

   #203695

   Purpose

   expresses ATP10B

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTEE

   Plasmid

   #159748

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

    Plasmid

    #22034

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450M.Rac1 s…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pTUU

    Plasmid

    #159749

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-NCBD + ACTR

    Plasmid

    #99335

    Purpose

    Bacterial coexpression of CBP NCBD and NCoA3 binding domains

    Depositor

    Peter Wright

    Article

    Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

    Inserts

    CBP NCBD (2059-2117) (CREBBP Mouse)
    SRC3 (1023-1093) (NCOA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3322_pETcon_SARS2_Omicron-BA2

    Plasmid

    #184409

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-PRKD1

    Plasmid

    #116778

    Purpose

    Lentiviral expression of PRKD1

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PRKD1 (PRKD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-STK31

    Plasmid

    #116796

    Purpose

    Lentiviral expression of STK31

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    STK31 (STK31 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-Sec22b-P33-shR

    Plasmid

    #208359

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations (shRNA resistance), 33 aa p…

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

    Plasmid

    #22023

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450A.Rac1 s…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid

    #69117

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    LL (566-567aa) mutated to AA. Silent mutations i…

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. SOX2 KO (T2A-H2B-EGFP)

    Plasmid

    #167969

    Purpose

    SOX2 CDS replacement with T2A-H2B-EGFP

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 2 (SOX2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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