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Myc-PSFΔRRM1

Plasmid

#35376

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-365

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Suv39H1_2

Plasmid

#36343

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR-RNF40i1

Plasmid

#59594

Purpose

Expression of shRNA against human RNF40

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

RNF40 (RNF40 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
GluR2_adRNA(1,20,6)-ADAR2

Plasmid

#124620

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-HA GCNF

Plasmid

#16336

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Insert

GCNF (Nr6a1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

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RapR-Shp2-mVenus-flag

Plasmid

#188655

Purpose

Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Shp2

Tags

Flag and mVenus

Expression

Mammalian

Mutation

iFKBP insertion at V406 with deletion of residues…

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDM1082

Plasmid

#108982

Purpose

Knockout cassette G418

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette G418

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-hActin-luc2

Plasmid

#48081

Purpose

Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Luciferase

Expression

Mammalian

Mutation

removed hsp70 promoter, inserted amplified 615 bp…

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pTP443

Plasmid

#216361

Purpose

xNup98-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup98-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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rNrx1beta(47-299)

Plasmid

#20173

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neurexin 1 beta (Nrxn1 Rat)

Tags

6X His tag

Expression

Insect

Mutation

no splice site

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pQTEV-VPS28

Plasmid

#34803

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

vacuolar protein sorting 28 (yeast) (VPS28 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pPnos (GB0072)

Plasmid

#68166

Purpose

Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Nos promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIN X2 DEST (w310-1)

Plasmid

#17399

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral; Destination vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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hADAM10 siRNA pSIREN-Retro-Q

Plasmid

#19140

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 siRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GWF196

Plasmid

#133613

Purpose

NS3aH1-VPR

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

NS3aH1-VPR

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-mNeptune2

Plasmid

#104012

Purpose

Plasmid encoding mNeptune2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mNeptune2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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