We narrowed to 15,172 results for: Egf
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Plasmid#132397Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1058AAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5
Plasmid#208377PurposeEpisomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoringDepositorTypeEmpty backboneExpressionMammalianPromoterhuman CMVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2
Plasmid#220508PurposeTGFb-inducible fluorescent reporter (TIE:EGFP)DepositorInsertsTGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal
UseZebrafish expression (tol2)TagsEGFPAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti
Plasmid#135955PurposeNeuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) N-terminal
UseLentiviralExpressionMammalianPromoterhSynapsinAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag
Plasmid#132400Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121AAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500
Plasmid#45788DepositorInsertdeGFP
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterpBAD-tetO1Available SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A
Plasmid#127660PurposeOver-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227ADepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct
Plasmid#175554PurposeTargeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.DepositorInsertNANOG
UseMouse TargetingTagsFKBPV-HA2-P2A-eGFPExpressionMammalianMutationnoneAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag
Plasmid#132410Purposeoverexpression in human cellsDepositorInsertdEsCas13b
UseLentiviralTagsflagExpressionMammalianMutationR295A; H300A; R849A; H854AAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)
Plasmid#191159PurposeExpression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry outputDepositorInsertmTagBFP:EGFP.s2:mCherry
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH
Plasmid#131683PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1DepositorInsertsEGFP-KASH
SpCas9 sgRNA vs mouse GRID1
UseAAVTagsKASHPromoterEF1a and mU6Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein
Plasmid#208378PurposeEpisomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split inteinDepositorTypeEmpty backboneExpressionMammalianPromoterhuman CMVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)
Plasmid#206434PurposeVasa 463-661 expression in Schneider cells for imagingDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174529PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertsPylS
PylT
TagsFlagExpressionMammalianPromoterU6Available SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag
Plasmid#132409Purposeoverexpression in human cellsDepositorInsertdRanCas13b
UseLentiviralTagsflagExpressionMammalianMutationH147A; H1044AAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)
Plasmid#208289PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA; mutations in iSpyMacPromoterCMV and T7Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)
Plasmid#198035PurposeUsed for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)
Plasmid#191164PurposeSimilar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequencesDepositorInsertmCherry:s70587:EGFP (additional cutsites)
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only