We narrowed to 9,647 results for: bli

1. pJBJZ_006

   Plasmid

   #155359

   Purpose

   Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pFP.R272

   Plasmid

   #138221

   Purpose

   Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgRNA(MS2)_zeo insert c-MYC

   Plasmid

   #164636

   Purpose

   sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

   Depositor

   Günter Schneider

   Insert

   target sequence for c-Myc activation (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RabV CVS-N2c(deltaG)-GCaMP6f

   Plasmid

   #73466

   Purpose

   Expresses GCaMP6f for calcium imaging

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   GCaMP6f
   

   Use

   Neurotropic virus

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Reza Zolfaghari, Catharine Ross

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pALS3-Ma-SUMO-TAG35-sfGFP

   Plasmid

   #212120

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   SUMO-TAG35-sfGFP
   M. alvus Pyl-tRNA
   

   Tags

   His6 and SUMO-TAG35

   Expression

   Bacterial

   Mutation

   E35TAG (within SUMO encoding sequence)

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLSU4cg10

   Plasmid

   #126078

   Purpose

   Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Promoter

   longCaMV35S

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TAF1

   Plasmid

   #39118

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TAF1 (TAF1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pFP.R364

   Plasmid

   #138234

   Purpose

   Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFP.R274

    Plasmid

    #138223

    Purpose

    Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AICSDP-144:CTCF-mEGFP

    Plasmid

    #168799

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

    Depositor

    Allen Institute for Cell Science

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. AAV-SYN-TdTomato-RAB11

    Plasmid

    #203732

    Purpose

    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV/EF1a-DIO-SCLM

    Plasmid

    #216759

    Purpose

    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    EF1a (human elongation factor 1 alpha)

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

    Plasmid

    #191675

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6X his and flag

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. attB53-Pac-attB53

    Plasmid

    #183610

    Purpose

    RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    Pac
    

    Use

    Rmce

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MECP2 - R133C in pENTR

    Plasmid

    #216594

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Mutation

    R133C

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE80L TriCatcher-RGDSP

    Plasmid

    #112631

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central RGDSP integrin-binding sequence

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

    Plasmid

    #73475

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-GFP-P2A-N2c(G)
    

    Use

    AAV

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

    Plasmid

    #110376

    Purpose

    Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFP.R275

    Plasmid

    #138224

    Purpose

    Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge