We narrowed to 31,171 results for: LIS

1. Lenti-sgNotch2#2/Cre

   Plasmid

   #193226

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgNotch2#2 (Notch2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCardinal-Rab4a-7

   Plasmid

   #56174

   Purpose

   Localization: Ras GTPase, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Rab4a (RAB4A Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

   Plasmid

   #167849

   Purpose

   Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10, nsp16
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMS04 [myo-3p::bPGC::SL2::mCherry]

   Plasmid

   #168172

   Purpose

   Expression of bPGC in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   bPGC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. YES1 (T348I) in pLX303

   Plasmid

   #42564

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YES1 (YES1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T348I

   Promoter

   CMV

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLY305

   Plasmid

   #184159

   Purpose

   Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   mutsfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   K140N, Mutations D10A and H840A on WT cas9. Synon…

   Promoter

   Pcon, PpspA-zraP-22, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCardinal-H3-23

   Plasmid

   #56163

   Purpose

   Localization: Nucleus/Histones, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   H3 (H3C2 Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pSUMO His6 SUMO SnRK2.2 (20-362)

   Plasmid

   #177845

   Purpose

   Bacterial Expression of SnRK2.2

   Depositor

   Karsten Melcher

   Article

   Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV_Sox2 HMEJ donor

   Plasmid

   #97322

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Sox2 HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. KLC1-Myc

    Plasmid

    #166962

    Purpose

    Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1 (Klc1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT-HGP-v3-NbVHH05-hIgG

    Plasmid

    #171565

    Purpose

    Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
    

    Use

    Tags

    BiP signal peptide and hIgG1-hinge-N297A-TEV site…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

    Plasmid

    #91882

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1+_LN-HA-AGO2-5xA

    Plasmid

    #92010

    Purpose

    Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP Kind2 S181E

    Plasmid

    #105312

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP Kind2 S181A

    Plasmid

    #105307

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. aav-U6-broccoli-hsp68-mCherry-MCS-pA

    Plasmid

    #195411

    Purpose

    AAV9 based enhancer activity assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid

    #176149

    Purpose

    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

    Promoter

    CMV

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge