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pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

Plasmid

#182355

Purpose

mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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MTK3_008

Plasmid

#123723

Purpose

Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS-VP16-EL222

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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3535 pET15b Bid p22

Plasmid

#8784

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p22 (Bid Mouse)

Tags

His

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCZGY1026 COX8aN::miniSOG in pENTR[1-2]

Plasmid

#66752

Purpose

miniSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

COx8aN (N terminal 29 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGG184

Plasmid

#165604

Purpose

Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

'CAGCG' PAM replacing the 'CGGCG&#…

Promoter

lac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_2

Plasmid

#91160

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR(T/A)

Plasmid

#118473

Purpose

Negative-control mutant for blueCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABEmax7.10HD-BlastR

Plasmid

#176477

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABEmax7.10HD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJG363

Plasmid

#91183

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG370

Plasmid

#91187

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gRNActrl+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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3495 pcDNA3 Bid p15

Plasmid

#8780

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p15 (Bid Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPIGA-PHD

Plasmid

#26778

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PGK-Hyg-p(A) (PGK1 )

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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MTK3_009

Plasmid

#123724

Purpose

Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PHYB-VP16-NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV Aquamarine-superYFP tandem

Plasmid

#157776

Purpose

Produces a fusion between Aquamarine and superYFP. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Tags

Aquamarine and superYFP

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Sox1

Plasmid

#61545

Purpose

Expresses Sox1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Sox1 (Sox1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A0801

Plasmid

#91022

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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