Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 159,879 results for: ins

Clear keywords
Showing: 7641 - 7660 of 159879 results

  1. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

    Enlarge

  12. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid
    #127239
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger2-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127239-plasmid-map-sequence-id-247086

    Enlarge

  13. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

    Enlarge

  14. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

    Enlarge

  16. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200403
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200403-plasmid-map-sequence-id-396808

    Enlarge

Showing: 7641 - 7660 of 159879 results