We narrowed to 28,533 results for: MUT

1. pTE3320

   Plasmid

   #107529

   Purpose

   Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hLbCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1Xpress-BcrGAP R1090A

   Plasmid

   #38176

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
   

   Tags

   Xpress

   Expression

   Mammalian

   Mutation

   aa 1004-1271 with R1090A point mutation

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

   Plasmid

   #68346

   Purpose

   Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   3xgRNA;PTENA, p53B,SMAD4A
   FlPO
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-evoSpCas9

   Plasmid

   #126765

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTAMAHISTEV_PfeAR480A-Q482A

   Plasmid

   #128946

   Purpose

   expresses R480A Q482A mutant of PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75), …

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-CDC42BPB__RGS6

   Plasmid

   #116810

   Purpose

   Lentiviral expression of CDC42BPB__RGS6 fusion

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CDC42BPB__RGS6
   

   Use

   Lentiviral

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-CDC42BPB__SYNJ2BP

   Plasmid

   #116811

   Purpose

   Lentiviral expression of CDC42BPB__SYNJ2BP fusion

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CDC42BPB__SYNJ2BP
   

   Use

   Lentiviral

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShuttle-EGFP-CLASP2 340-1084 9xS/A

   Plasmid

   #24384

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) 9xS/A (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. hnRNPA2_LC_P298L_S285C

   Plasmid

   #104469

   Purpose

   express His tagged P298L S285C hnRNPA2 LC

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   P298L S285C

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TAOK1__PIPOX

    Plasmid

    #116839

    Purpose

    Lentiviral expression of TAOK1__PIPOX fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    TAOK1__PIPOX
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. SLC30A10-delta105-107

    Plasmid

    #65205

    Purpose

    Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta 105-107 amino acids

    Promoter

    CMV

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

12. CRY-GalVP16l (B694)

    Plasmid

    #92030

    Purpose

    expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    GalBD-VP16

    Expression

    Mammalian

    Mutation

    NLS sequence of CRY2 mutated

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. G766E EPHA3 pcDNA3.1

    Plasmid

    #102753

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    G766E (GGA > GaA)

    Promoter

    CMV

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-2B-Nkx2-5 delta HD-G8

    Plasmid

    #98614

    Purpose

    Gateway entry vector NKX2-5 _HD

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 _HD (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Mutation

    Deletion of the homeodomain. Replaced by 8 glycin…

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF5/FRT/DEST-3xHA/mGli2 P5-6A

    Plasmid

    #51253

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

16. CMV-10-3xFLAG-LGP2-MIII

    Plasmid

    #58683

    Purpose

    Expresses LGP2 with a mutation to motif III in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    LGP2-MIII (DHX58 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    threonine and serine of helicase motif III mutate…

    Promoter

    CMV

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Rb-379-928Δ703-737-pGex2T

    Plasmid

    #21933

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928D703-737 o…

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZS1[GalS-T,TreR-T]

    Plasmid

    #60775

    Purpose

    Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    GalS-T
    TreR-T
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. TAN DBD with GalS LB…

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZS1[RbsR-T,TreR-T]

    Plasmid

    #60776

    Purpose

    Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    RbsR-L
    TreR-T
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. TAN DBD with RbsR LB…

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mChF-AIP (TD)

    Plasmid

    #61529

    Purpose

    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    FK506 binding protein 1A (FKBP1A Human)
    

    Tags

    AIP with TD mutation (see comments) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only