We narrowed to 8,793 results for: Set;

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pSBK.010

Plasmid

#187210

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo-W35A

Plasmid

#179397

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo(W35A)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W35A

Promoter

CAGGS

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_3EGFP

Plasmid

#185967

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-Egfp cassette in forward direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo-W42A

Plasmid

#179394

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo(W42A)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W42A

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pv7_MBD1_MBD_noEGFP

Plasmid

#179406

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Tags

AviTag

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pv7_CBX1_2xChromo_noEGFP

Plasmid

#179395

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo_noEGFP

Tags

AviTag

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD-R22A

Plasmid

#179405

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD(R22A)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R22A

Promoter

CAGGS

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

Plasmid

#169665

Purpose

Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression

Expression

Mammalian

Promoter

PhCMV / PPGK / RPBSA

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2333_Tier3(Lenti_inverse)-YPet_Puro

Plasmid

#169664

Purpose

Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven YPet and PuroR expression

Expression

Mammalian

Promoter

PhCMV / RPBSA

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2304-Tier1-Tactb-PPGK-NLuc_TB

Plasmid

#169549

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Expression

Mammalian

Promoter

PPGK

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-tetO-mCherry-attB53

Plasmid

#183612

Purpose

RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tetO-mCherry

Use

Rmce

Promoter

tetO

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179075

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179050

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179049

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179048

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179047

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179046

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179045

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-ATG8

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179044

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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