We narrowed to 13,612 results for: NSI
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Plasmid#236185PurposeThe plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.DepositorInsertQuorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
PromoterQuorum sensing promoterAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(C)
Plasmid#236043PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-empty
Plasmid#236037PurposeThe plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXK-1/1
Plasmid#91539PurposeProtein expression and purification of human SH3 domain construct TXK-1/1DepositorInsertTXK-1/1 (TXK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
HR-TERT-promotor
Plasmid#71398PurposeHomologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
PromoterEndogenous TERT promotorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6-CjCas9-MS2gRNA-Backbone
Plasmid#210711PurposeThis plasmid express MS2 loop containing Cj Cas9 gRNADepositorInsertMS2 loop containing Cj Cas9 gRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralPromoterEFS promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA
Plasmid#159283PurposeA multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).DepositorInsertsHuman INS sgRNA
Human INS guide RNA
ExpressionMammalianPromoterU6Available SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG
Plasmid#174892Purposeamber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_SZ1-RGG-GFP-RGG
Plasmid#124932PurposeExpression of SZ1-RGG-GFP-RGGDepositorAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
ExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_LAMP1
Plasmid#111669PurposeMAC-tagged gene expressionDepositorInsertLAMP1 (LAMP1 Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AK3 Hyg
Plasmid#37539DepositorAvailable SinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GPI
Plasmid#203759PurposeEncodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJA103
Plasmid#128519PurposeExpresses human amyloid Lysozyme in Pichia pastorisDepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHW110031
Plasmid#222437PurposeLevel 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.DepositorInsertCBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-4mtD3cpv
Plasmid#181874PurposeExpresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoterDepositorInsert4mtD3cpv
UseAAV and AdenoviralExpressionMammalianMutationthe 4mtD3cpv CDS differs from the recorded GeneBa…PromoterCaMKIIaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
FMRP shRNA
Plasmid#206074Purposeexpression of FMRP RNA for gene silencing in mammalian cellsDepositorAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only