We narrowed to 807 results for: Numb

1. pQCXIB Flag MFF S155A

   Plasmid

   #74385

   Purpose

   Retroviral expression vector containing Flag tagged S155A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 155 Ala (numbering based on MFF isoform 1)

   Promoter

   CMV

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pQCXIB Flag MFF S172A

   Plasmid

   #74386

   Purpose

   Retroviral expression vector containing Flag tagged S172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   CMV

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCS2-mkgDUX4mal*leu*

   Plasmid

   #21173

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   First point mutation at nucleotide 5114 (numberin…

   Promoter

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pHsSyx3-MBP

   Plasmid

   #179286

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   TEV protease cleavage site + mature maltose bindi…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBP-HsSyx3

   Plasmid

   #179287

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + mature maltose binding protein + TEV prote…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-HsSyx-375-792

   Plasmid

   #179291

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + mature maltose binding protein + TEV prote…

   Expression

   Bacterial

   Mutation

   residues 375-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RMCE wild type DNA-PKcs

   Plasmid

   #233911

   Purpose

   Low copy number provides expression of human DNA-PKcs

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   dna-pkcs cDNA (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-EGFP-Tau AP

   Plasmid

   #46905

   Purpose

   expresses EGFP tagged Tau AP in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. RMCE halo-flag DNA-PKcs

   Plasmid

   #233918

   Purpose

   Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   flag-halo

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-MYO5C

    Plasmid

    #135404

    Purpose

    Expresses GFP-tagged human MYO5C in mammalian cells.

    Depositor

    Richard Cheney

    Article

    Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

    Insert

    MYO5C (MYO5C Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Relative to the GenBank MYO5C cDNA sequence repor…

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K3753R

    Promoter

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS1951

    Plasmid

    #105868

    Purpose

    High copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Hspa1a (synonym Hsp68) MiniPromoter (HSPA1A, HSPA1A Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Hsp68 MiniPromoter

    Available Since

    Feb. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCJ208

    Plasmid

    #162681

    Purpose

    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of W185-F191 with G251-T472 from MHET…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Zebrafish ArcLight Q175

    Plasmid

    #53616

    Purpose

    Genetically encoded voltage sensor ArcLight with improved kinetics

    Depositor

    Vincent Pieribone

    Article

    Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

    Insert

    Zebrafish ArcLight-Q175 (tpte Zebrafish, Aequorea victoria, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dr-VSD contains R153Q mutation and an amino acid …

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJ209

    Plasmid

    #162682

    Purpose

    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of G251-T472 with W185-F191 from PETa…

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTT5-hSDC1-2

    Plasmid

    #52378

    Purpose

    expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GAGAL of syndecan-1 replaced with of GADED of SDC…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1 - HA-KLF4 FL delta K5S

    Plasmid

    #34594

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deletion of K5S, corresponding to bases 1781 to 2…

    Promoter

    CMV

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pTT5-hSDC1-4

    Plasmid

    #52375

    Purpose

    expresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GAGAL of syndecan-1 replaced with GDLDD of SDC4; …

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBpuro - HA KLF4 FL delta K5S

    Plasmid

    #34592

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deletion of K5S, corresponding to bases 1781 to 2…

    Promoter

    LTR

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

    Plasmid

    #231233

    Purpose

    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TAD deleted (delta1-143); numbering based on sequ…

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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