We narrowed to 886 results for: nanobody

1. pScarlessHD-N-3xVHH05-DsRed

   Plasmid

   #171581

   Purpose

   For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3xVHH05 (UBE2J1 Human)
   

   Expression

   Insect

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScarlessHD-N-3x127D01-DsRed

   Plasmid

   #171579

   Purpose

   For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3x127D01 (CXCR2 Human)
   

   Expression

   Insect

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBC2T-actin

   Plasmid

   #108968

   Purpose

   expresses BC2-tag-actin in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   beta-actin (ACTB Human)
   

   Tags

   BC2-tag

   Expression

   Mammalian

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBC2T-lamin

   Plasmid

   #108972

   Purpose

   expresses BC2-tag-lamin in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   lamin B1 (LMNB1 Human)
   

   Tags

   BC2-tag

   Expression

   Mammalian

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

   Plasmid

   #160742

   Purpose

   Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4,K852A)
   

   Tags

   HA-Tag

   Expression

   Mammalian

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOD2087-trnDEG

   Plasmid

   #89369

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Promoter

   Pmec-18

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-CCre-MBP4-WPRE

   Plasmid

   #193919

   Purpose

   Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   CCre-MBP4
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-NCre-MBP5-WPRE

   Plasmid

   #193918

   Purpose

   Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   NCre-MBP5
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192788

   Purpose

   aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody enhancer
   

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-CCre-MBP1-WPRE

    Plasmid

    #193917

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP1
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-NCre-MBP6-WPRE

    Plasmid

    #193916

    Purpose

    Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP6
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

    Plasmid

    #242589

    Purpose

    A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh (actin nanobody)
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-HAPPID1

    Plasmid

    #204626

    Purpose

    Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.

    Depositor

    James McDonnell

    Article

    Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

    Inserts

    HAPPID1 heavy chain
    HAPPID1 light chain
    

    Expression

    Mammalian

    Promoter

    mouse elongation factor 1 alpha and rat elongatio…

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIP-SHMY-A1Pi

    Plasmid

    #182648

    Purpose

    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    A1Pi (SERPINA1 Human)
    

    Use

    Retroviral

    Tags

    His6-tag, N-terminal ER targeting sequence of hem…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

    Plasmid

    #205283

    Purpose

    GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)

    Depositor

    Matthias Prigge

    Insert

    GCaMP8f
    

    Use

    AAV

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin nanobody
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEos2-dVHH[GFP] in pCSDEST

    Plasmid

    #162870

    Purpose

    Super-resolution of GFP in culture

    Depositor

    Rob Parton

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    mEos2-dVHH[EGFP]
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid

    #158133

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-TetRVP64
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid

    #158135

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-Gal4VP64
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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