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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,392 results for: crispr grnas

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Showing: 7781 - 7800 of 8392 results

  7. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    TU-sgRNA - L1/RE
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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  11. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  12. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

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  17. pSJ107-2x

    Plasmid
    #208812
    Purpose
    Expresses SoxS activation domainand gRNA targeting two copies of 107 region within the synthetic promoter in bacterial cell
    Depositor
    Marc Erhardt
    Article
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    sgRNA 107-2x_mRFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    CRISPR/dCas9-responsive synthetic promoter
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208812-plasmid-map-sequence-id-418905

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  18. pSJ106-2x

    Plasmid
    #208860
    Purpose
    Expresses SoxS activation domain and gRNA targeting two copies of 106 region within the synthetic promoter in bacterial cell
    Depositor
    Marc Erhardt
    Article
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    sgRNA 106-2x_mRFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    CRISPR/dCas9-responsive synthetic promoter
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208860-plasmid-map-sequence-id-415439

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  19. B52_puro_gRNA_3418711_KCNB2

    Plasmid
    #197558
    Purpose
    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)
    Depositor
    Darius Bägli
    Insert
    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    Use
    Grna with puromycin selection
    Tags
    puromycin resistance cassette under CMV promotion
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197558-plasmid-map-sequence-id-388709

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Showing: 7781 - 7800 of 8392 results