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PM18033-PLS-KP1_rpoA-U

Plasmid

#190975

Purpose

Test the U-to-C editing activity of KP1 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP1

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEL638

Plasmid

#189831

Purpose

The T-DNA backbone of nCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-empty

Plasmid

#221239

Purpose

Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-4xFKBP scaffold

Use

Retroviral

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAB082h

Plasmid

#79240

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23C

Expression

Bacterial

Promoter

Ppro1

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-mOvol1

Plasmid

#120359

Purpose

Gateway entry vector containing mouse Ovol1.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

Ovol1 (Ovol1 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tet-on and CBh

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBlue-loxPuro-APEX-PML_LASA

Plasmid

#140273

Purpose

a targeting plasmid for APEX-PML

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Promoter

endogenous

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mOrange

Plasmid

#134298

Purpose

doxycycline-inducible expression of mouse mOrange in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

mOrange

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6_rpoA-C

Plasmid

#190966

Purpose

Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CMV+ plasmid with onboard mCherry cassette

Plasmid

#169741

Purpose

CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-EFS:GFP

Plasmid

#68484

Purpose

Retrovirus expressing GFP driven by EFS promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

EFS and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pT2ADW_2paRAF

Plasmid

#129654

Purpose

For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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3924-55

Plasmid

#176499

Purpose

pTRE-EGFP-T2A-TFEB(S3E)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(human; full-length; S3E mutation)-

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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3923-51

Plasmid

#176498

Purpose

pTRE-EGFP-T2A-TFEB(S3A)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(human; full-length; S3A mutation)

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-Gal4DBD-CapN-SsrA-CapC

Plasmid

#213533

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-PLAGL2

Plasmid

#133380

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pet 3d alpha 1 beta 2 CP

Plasmid

#13450

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pPaxillin-TagRFP675-N1

Plasmid

#44276

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

chicken paxillin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Oct. 3, 2013

Availability

Academic Institutions and Nonprofits only

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