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pHK001.1

Plasmid

#235449

Purpose

NOT gate receiver with binding site (bs) for sgRNA1

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK001.2

Plasmid

#235450

Purpose

NOT gate receiver with bs for sgRNA2

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.3

Plasmid

#235451

Purpose

NOT gate receiver with bs for sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.4

Plasmid

#235452

Purpose

NOT gate receiver with bs for sgRNA4

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.5

Plasmid

#235453

Purpose

NOT gate receiver with bs for sgRNA5

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.6

Plasmid

#235454

Purpose

NOT gate receiver with bs for sgRNA6

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOT gate

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-L7-6-DD-T6B-WT-YFP

Plasmid

#235145

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-WT-YFP

Plasmid

#235141

Purpose

Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v10 bacteria

Plasmid

#208594

Purpose

Production and purification of catalytically active SynCas.v10

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

SynCas.v10

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria

Plasmid

#208595

Purpose

Production and purification of catalytically inactive SynCas.v10

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

dSynCas.v10

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

Plasmid

#208596

Purpose

Production and purification of catalytically active SynCas.v12

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

SynCas.v12

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

Plasmid

#208597

Purpose

Production and purification of catalytically inactive SynCas.v12

Depositor

Omar Akbari

Article

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

dSynCas.v12

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEt28a-SilkTag-CBM-H6

Plasmid

#230903

Purpose

Bacterial expression of SilkTag

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

SilkTag-CBM-H6

Tags

HisTag

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
dimerTomato-wGBD

Plasmid

#129635

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

Cdc42 binding domain of N-WASP

Expression

Mammalian

Promoter

CMVdel

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-MET25pr

Plasmid

#221100

Purpose

Methionine-repressible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-MET25pr

Plasmid

#221103

Purpose

Methionine-repressible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-CUP1pr

Plasmid

#221106

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POSV801-SP44-SnaAinBCDEOT1T2

Plasmid

#225056

Purpose

Heterologous expression in Streptomyces with inactive SnaA

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaA

Expression

Bacterial

Mutation

S556A/S1616A

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pmirGLO-mir1-8mer4x (pEZ184)

Plasmid

#229058

Purpose

Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-1

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

Human miR-1 binding sites

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge