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Lenti-sgNotch3#1/Cre

Plasmid

#193227

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch3 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch3#1 (Notch3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ATX3-JD

Plasmid

#184247

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

Depositor

Sandra Macedo Ribeiro

Article

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 Josephin domain (ATXN3 Human)

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Premature stop codon V183X

Promoter

T7 promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996

Plasmid

#171131

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluSz 3501-4065

Plasmid

#171132

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluY WT 2598-2996

Plasmid

#171134

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066

Plasmid

#171136

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-CD45

Plasmid

#175943

Purpose

knockout mouse CD45

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD45 gRNA (Ptprc Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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330-BFP-hCYP3A4-enhancer-R-sgRNA

Plasmid

#176841

Purpose

Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer right boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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330-cherry-hCYP3A4-enhancer-L-sgRNA

Plasmid

#176840

Purpose

Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer left boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93

Plasmid

#158629

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Mutation

E93Q

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_Donor/Reporter-COL4A3

Plasmid

#130279

Purpose

COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A3_self-cleavingCas9

Plasmid

#130281

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A5_self-cleavingCas9

Plasmid

#130282

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

Plasmid

#154778

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148

Plasmid

#158630

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Mutation

Q148A

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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