We narrowed to 19,051 results for: Tec

1. Lenti AID-BEmax

   Plasmid

   #157950

   Purpose

   Lentiviral expression of AID-BEmax

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Lenti AID-BEmax
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK-IKZF3-CTERM-GFP

   Plasmid

   #185779

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, IKZF3 minidegron

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PIK3C2B gRNA (BRDN0001149112)

   Plasmid

   #76713

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SFFV-IKZF3-CTERM-GFP

   Plasmid

   #185769

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, IKZF3 minidegron

   Mutation

   none

   Promoter

   SFFV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRB133

   Plasmid

   #180176

   Purpose

   GluN2A in pCDNA3.1

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN2A (Grin2a Rat)
   

   Expression

   Mammalian

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

   Plasmid

   #193849

   Purpose

   Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd-V106W
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGK-AID-CTERM-GFP

   Plasmid

   #185778

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, AID

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. shMEK1/2 puro

   Plasmid

   #72567

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniABEmax (pRZ900)

   Plasmid

   #131311

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SFFV-DTAG-CTERM-GFP

    Plasmid

    #185765

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-His6MBP-A3A-Gly6His6

    Plasmid

    #187822

    Purpose

    Expression human APOBEC3A in E.coli.

    Depositor

    Hao Wu

    Article

    Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

    Insert

    APOBEC3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter (on the backbone)

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8.13-m

    Plasmid

    #136296

    Purpose

    expresses ABE8.13-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. shMEK1/2 v2 puro

    Plasmid

    #72568

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_eA3A-NG-nCas9

    Plasmid

    #163536

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-HBO1-G485A pc2201

    Plasmid

    #167570

    Purpose

    The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

    Insert

    histone acetyltransferase 1 (KAT7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G485A

    Promoter

    CMV

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti PA-mCit

    Plasmid

    #113451

    Purpose

    Lentiviral ProteinA-mCitrine expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUBbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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