We narrowed to 15,726 results for: NOL

1. RS18965_pMQ30_KO_Kan

   Plasmid

   #185394

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18965 upstream flanking homology region
   RR42_RS18965 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RS18970_pMQ30_KO_Kan

   Plasmid

   #185395

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18970 upstream flanking homology region
   RR42_RS18970 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

   Plasmid

   #200124

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

   Plasmid

   #200125

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmCherry-TdIF2

   Plasmid

   #196906

   Purpose

   visualization of TdIF2/ERBP as a fusion to mCherry

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   TdIF2
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHcRed-CLCa

   Plasmid

   #196911

   Purpose

   visualization of clathrin-coated vesicle in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

   Insert

   Clathrin-Coated light chain
   

   Tags

   HcRed

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-TdIF2

   Plasmid

   #199699

   Purpose

   visualization of nucleolus in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

   Insert

   TdIF2
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

   Plasmid

   #192399

   Purpose

   Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-Rluc-FRT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

   Plasmid

   #192398

   Purpose

   On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-Rluc-FRT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

    Plasmid

    #192373

    Purpose

    To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-TB-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

    Plasmid

    #192372

    Purpose

    To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192404

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192401

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192400

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge