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pWZL Neo Myr Flag OXSR1

Plasmid

#20553

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

OXSR1 (OXSR1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pENTR1A_mmFbxw7a (closed)

Plasmid

#160101

Purpose

Expresses murine Fbxw7 alpha in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75243

Purpose

CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag ULK4

Plasmid

#20662

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ULK4 (ULK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag RPS6KL1

Plasmid

#20626

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KL1 (RPS6KL1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBABE MDproER

Plasmid

#13495

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1, ESR1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD (containing the point mutation A…

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag STK32B

Plasmid

#20639

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

STK32B (STK32B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pMIR-REPORT-MYC CDS_WT

Plasmid

#108924

Purpose

MYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vector

Depositor

Jianjun Chen

Article

Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

Insert

MYC (MYC Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TESK1

Plasmid

#20650

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TESK1 (TESK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-KS

Plasmid

#136617

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4-IRES-Sox2 (Klf4, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 E76G

Plasmid

#140862

Purpose

expresses PTPN11 E76G

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Expression

Mammalian

Mutation

E76G

Promoter

EF1a

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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sgALK(Chr. Inv.)

Plasmid

#85569

Purpose

Expresses sgRNA targeting human ALK between exon 20 and 21

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

anaplastic lymphoma receptor tyrosine kinase (ALK Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pVZ_PcoaT_SQE1

Plasmid

#103860

Purpose

Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803

Depositor

Ilka Axmann

Article

Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

Insert

squalene epoxidase 1 (XF1 Mustard Weed)

Use

Synthetic Biology

Expression

Bacterial

Mutation

inserted gene is codon optimized. 2nd amino acid …

Promoter

PcoaT (from Synechocystis sp. PCC 6803)

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag NME7

Plasmid

#20509

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NME7 (NME7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
sgEGFR(B)

Plasmid

#85565

Purpose

Expresses sgRNA targeting human EGFR around codon T790

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

epidermal growth factor receptor (EGFR Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

Plasmid

#105806

Purpose

Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-Cerulean; ORF2: TEV-Amber

Expression

Mammalian

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75242

Purpose

CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PAPSS1

Plasmid

#20557

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PAPSS1 (PAPSS1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST flag-CAD R2187A

Plasmid

#169827

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

R2187A, T456A S1406A S1859A; TCCC -> AGTC sile…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD H1734A H1741A

Plasmid

#169830

Purpose

Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

H1734A H1741A; TCCC -> AGTC silent mutations a…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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