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Showing: 91 - 120 of 148 results

  1. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  3. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  4. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  5. pDule2 - Mj Acd A9

    Plasmid
    #197652
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197652-plasmid-map-sequence-id-388215

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  6. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  7. pDule- Mj Acd A9

    Plasmid
    #197651
    Purpose
    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
    Depositor
    Ryan Mehl
    Article
    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-
    Inserts
    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32A, L65D, F108L, Q109E, D158S, L162T
    Promoter
    GlnS and lpp
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197651-plasmid-map-sequence-id-389657

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  8. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

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  9. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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  11. Ma-Barnase

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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  12. Ma-pREP

    Plasmid
    #197571
    Purpose
    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    111TAG and 8TAG/112TAG
    Promoter
    AraC, T7, cat promoter (constitutive), and lpp (c…
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197571-plasmid-map-sequence-id-389424

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  13. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

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  14. CMV-SEAP

    Plasmid
    #24595
    Depositor
    Alan Cochrane
    Insert
    SEAP (ALPP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 23, 2010
    Availability
    Academic Institutions and Nonprofits only
    24595-plasmid-map-sequence-id-260470

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  26. pBS-V9

    Plasmid
    #173797
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173797-plasmid-map-sequence-id-342771

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  27. pBS-V7

    Plasmid
    #173796
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene latifolia clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173796-plasmid-map-sequence-id-342770

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  28. pBS-V6

    Plasmid
    #173795
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene conica clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173795-plasmid-map-sequence-id-342769

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  29. pBS-V4

    Plasmid
    #173794
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Nicotiana tabacum clpP1 gene with native regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173794-plasmid-map-sequence-id-342763

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  30. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Article
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

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Showing: 91 - 120 of 148 results