We narrowed to 24,075 results for: Spr

1. pSprayerHE

   Plasmid

   #165150

   Purpose

   Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Insert

   BASU-GAL4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACRISPR-CM

   Plasmid

   #203588

   Purpose

   A sgRNA expression plasmid for genome editing in Burkholderia

   Depositor

   Leonilde Moreira

   Article

   Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-PE SpRY

   Plasmid

   #179082

   Purpose

   All-in-one prime editor piggyBac transposon, SpRY variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

   Promoter

   CAG

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miniCRISPRoff-v1

   Plasmid

   #197030

   Purpose

   Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized miniCRISRPoff-v1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiCRISPRv2_sgCtrl#1

   Plasmid

   #174148

   Purpose

   Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPR_Puro_sgAAVS1

   Plasmid

   #187818

   Purpose

   pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSprayer

   Plasmid

   #165148

   Purpose

   Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Insert

   BASU-GAL4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPRi

   Plasmid

   #199808

   Purpose

   vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

   Depositor

   Steve Salipante

   Article

   Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

   Inserts

   dCas9
   sgRNA with GFP cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACRISPR-TP

    Plasmid

    #203589

    Purpose

    A sgRNA expression plasmid for genome editing in Burkholderia

    Depositor

    Leonilde Moreira

    Article

    Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPRv2_sgCtrl#2

    Plasmid

    #174149

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiCRISPRv1_SDHB

    Plasmid

    #177981

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHB (SDHB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL-CRISPR.EFS.PAC

    Plasmid

    #57828

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPRv2_PROX1_1+3

    Plasmid

    #121176

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpRY

    Plasmid

    #198544

    Purpose

    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPRv1_UQCRC2

    Plasmid

    #177982

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLSPR24

    Plasmid

    #217309

    Purpose

    Promoterless (SwaI Cutsite) mRuby3:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLSPR25

    Plasmid

    #217310

    Purpose

    Promoterless (SwaI Cutsite) mNeonGreen:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLSPR30

    Plasmid

    #217315

    Purpose

    GAL7(p)-mRuby3:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge