We narrowed to 1,707 results for: abat

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SEC13 in pRK5

Plasmid

#154215

Purpose

Untagged SEC13 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

Sec13

Tags

NONE

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-C3orf17-flag

Plasmid

#70646

Purpose

Expresses C3orf17 in mammalian cells with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C3orf17 (NEPRO Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-C9orf114-flag

Plasmid

#70647

Purpose

Expresses C9orf114 with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
HA GST F5A deltaCT S6K1 pRK5

Plasmid

#8467

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta CT p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2-E451A

Plasmid

#73674

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2-L261A

Plasmid

#73672

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
HA GST deltaCT S6K1 pRK5

Plasmid

#8468

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta CT p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9

Plasmid

#50661

Purpose

Doxycycline-inducible lentiviral expression of SpCas9

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Tet ON

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-fDIO-rtTA

Plasmid

#166598

Purpose

Encodes Flp-dependent rtTA

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

rtTA

Use

AAV

Promoter

human synapsin 1

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-fDIO-tTA

Plasmid

#166597

Purpose

Encodes Flp-dependent tTA

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

tTA

Use

AAV

Promoter

human synapsin 1

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD wt

Plasmid

#19316

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD wt

Plasmid

#19307

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #1

Plasmid

#26625

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1 Rheb1 shRNA #2

Plasmid

#26626

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pSAD-F3-NPEST-iCRE-2A-mCherryPEST

Plasmid

#99608

Purpose

Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

iCRE
mCherry

Use

Unspecified

Tags

PEST

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-S6K1

Plasmid

#100509

Purpose

Expresses FLAG tagged S6K1 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

S6K1 (Rps6kb1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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