We narrowed to 4,840 results for: pcr
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Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
ExpressionBacterialAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-TagGFP2-PuroR
Plasmid#80970PurposeCRISPaint gene taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagGFP2
UseGene taggingPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-6xGLY-FLAG-kanMX6
Plasmid#20751DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-6xGLY-His-tag-kanMX6
Plasmid#20763DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-LRP6c
Plasmid#42960PurposeExpresses optogenetically controlled LRP6 (C-terminal domain)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCry2PHR-mCherry-LRP6c
ExpressionMammalianMutationLPR6 cytoplasmic domain onlyPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS595 tet-hCMV-EGFPcre
Plasmid#11957DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-cre (cre bacteriophage P1)
UseCre/LoxExpressionMammalianAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-His3MX6
Plasmid#41598DepositorPublicationInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionYeastPromoterTEFAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-GFP(S65T)-kanMX6
Plasmid#39292DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP2A-clover-BlastR
UseCRISPRAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
ExpressionBacterialPromotertetR/pLtetOAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
XE18 Noggin-CSKA
Plasmid#16998DepositorInsertnoggin PCR clone (nog.L Frog)
UseXenopus expressionAvailable sinceMay 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/λ
Plasmid#52214PurposeHuman immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin lambda constant region
ExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorTypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_AAVS1-T2
UseCRISPRExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lox-Stop-Lox TOPO
Plasmid#11584PurposeCre-dependent activation of gene of interest by removing Stop sequences; Puro selection.DepositorInsertloxP-STOP-loxP
UseCre/LoxExpressionMammalianAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_GFP-T1
Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_GFP-T1
UseCRISPRExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_AAVS1-T1
UseCRISPRExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA_GFP-T2
Plasmid#41820PurposeExpresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineeringDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_GFP-T2
UseCRISPRExpressionMammalianAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBC-DT2T3
Plasmid#50591PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, U6-29t plus, U6-1p, T3
UseCRISPR; Pcr templateExpressionPlantAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by