We narrowed to 6,702 results for: pcr

1. pCR1056

   Plasmid

   #71824

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22B_PcrA_2Bchimera

   Plasmid

   #102998

   Purpose

   Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

   Depositor

   Martin Webb

   Insert

   PcrA_2Bchimera
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Bacilllus stearothermophilus PcrA in which the 2B…

   Promoter

   T7

   Available Since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR_eGFP_191

   Plasmid

   #111821

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 171-191
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR2.1_HBB_N

   Plasmid

   #87847

   Purpose

   pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

   Depositor

   Marina Kleanthous

   Article

   Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

   Insert

   Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPReporter

   Plasmid

   #65007

   Purpose

   E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAPcrRNA_Lmo

   Plasmid

   #126501

   Purpose

   L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcrRNA.con

   Plasmid

   #61285

   Purpose

   Constitutive repeat-only E. coli CRISPR array

   Depositor

   Chase Beisel

   Article

   Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR1164

   Plasmid

   #111100

   Purpose

   Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCD2 gRNA (FANCD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR_DMS3g24

   Plasmid

   #89244

   Purpose

   Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Insert

   CRISPR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR1054

    Plasmid

    #71822

    Purpose

    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFPcR5

    Plasmid

    #149484

    Purpose

    Broad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    PcaU(AM)-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3B5B

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR1075

    Plasmid

    #111093

    Purpose

    Expresses eGFP donor in pUC19

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-HA

    Plasmid

    #67171

    Purpose

    CRISPaint universal donor for HA fusion tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRRNAcos

    Plasmid

    #78492

    Purpose

    Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR1002

    Plasmid

    #71820

    Purpose

    Express Streptococcus pyogenes Cas9

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPyCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR_eGFP_369

    Plasmid

    #111823

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 349-369
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR1063

    Plasmid

    #111091

    Purpose

    Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    dCas9-KRAB
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR1003

    Plasmid

    #71821

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR

    Plasmid

    #113252

    Purpose

    pUC19 backbone with E. coli CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    E. coli CRISPR array
    

    Use

    Integration substrate

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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