We narrowed to 146 results for: single guide rna sgrna

1. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

   Plasmid

   #133334

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133344

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_cpaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

   Plasmid

   #133343

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133345

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

   Plasmid

   #133341

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-Cas9-2A-GFP-sgRNA

   Plasmid

   #124889

   Purpose

   Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

   Depositor

   Harvey Lodish

   Article

   Li et al Haematologica. 2016 Jun;101(6):e216-9. d

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   2A-GFP

   Expression

   Mutation

   Promoter

   MSCV

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiGuide

   Plasmid

   #117986

   Purpose

   pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

   Depositor

   Paul Khavari

   Article

   Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

   Insert

   sgRNA backbone (F+E)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - EGFP sgRNA 2

   Plasmid

   #51761

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR - EGFP sgRNA 4

    Plasmid

    #51763

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR - EGFP sgRNA 5

    Plasmid

    #51764

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psgRNA-2xMS2-lacO

    Plasmid

    #181909

    Purpose

    Single guide RNA with 2XMS2 loops targeting bacterial lac operator

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    sgRNA-2XMS2-lacO
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psgRNA-2xMS2-mSAT

    Plasmid

    #181908

    Purpose

    Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    sgRNA-2XMS2-mSAT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60228

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS1099: Dual-sgRNA.Design 4

    Plasmid

    #159537

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with two guide RNA cassettes
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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