We narrowed to 6,240 results for: cas9 expression plasmid

1. pML104-NatMx3

   Plasmid

   #83477

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   NatMx3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pSPneoHHgRNAH

   Plasmid

   #63557

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSPneogRNAH

   Plasmid

   #63556

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b-SpRY-His

   Plasmid

   #179320

   Purpose

   Plasmid for bacterial expression and purification of SpRY-Cas9

   Depositor

   Miguel Angel Moreno-Mateos, Julián Cerón

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-SpG-His

   Plasmid

   #179318

   Purpose

   Plasmid for bacterial expression and purification of SpG-Cas9

   Depositor

   Miguel Angel Moreno-Mateos, Julián Cerón

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpG
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRRNAcos

   Plasmid

   #78492

   Purpose

   Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

   Depositor

   David Bikard

   Article

   Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

   Insert

   tracrRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HCP3

   Plasmid

   #166105

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HCP7

   Plasmid

   #166109

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPEY-GAL-Nat

    Plasmid

    #232097

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML107-KAE1

    Plasmid

    #232891

    Purpose

    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    KAE1 gRNA (KAE1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML107-SAM50

    Plasmid

    #232895

    Purpose

    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    SAM50 gRNA (SAM50 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML107-ARB1

    Plasmid

    #232888

    Purpose

    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ARB1 gRNA (ARB1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pML107-RER2

    Plasmid

    #232884

    Purpose

    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    RER2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pML104-LEU2-3

    Plasmid

    #232894

    Purpose

    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pML104-HygMx4-URA3-3

    Plasmid

    #232886

    Purpose

    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pML104-HygMx4-URA3-2

    Plasmid

    #232885

    Purpose

    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pML107-AVO1

    Plasmid

    #232889

    Purpose

    Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    AVO1 gRNA (AVO1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pML107-YFR054C

    Plasmid

    #232900

    Purpose

    Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    YFR054C gRNA (YFR054C Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pML107-ZIM17

    Plasmid

    #232901

    Purpose

    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ZIM17 gRNA (ZIM17 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pML107-VHT1

    Plasmid

    #232898

    Purpose

    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    VHT1 gRNA (VHT1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pML107-SRP14

    Plasmid

    #232896

    Purpose

    Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    SRP14 gRNA (SRP14 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pML107-TLG1

    Plasmid

    #232897

    Purpose

    Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    TLG1 gRNA (TLG1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pML107-ADE13

    Plasmid

    #232887

    Purpose

    Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ADE13 gRNA (ADE13 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pML107-LAS17

    Plasmid

    #232892

    Purpose

    Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LAS17 gRNA (LAS17 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pML104-LEU2-2

    Plasmid

    #232883

    Purpose

    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pML107-INO80

    Plasmid

    #232890

    Purpose

    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    INO80 gRNA (INO80 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLII-CRISPR for CKA4

    Plasmid

    #238521

    Purpose

    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4

    Depositor

    Michael Hothorn

    Insert

    AtCK2alpha4 (AT2G23070 Mustard Weed)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. BB3nK_ext_AC

    Plasmid

    #108686

    Purpose

    Plasmid for assembly of donor DNA templates consisting of two expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BB3nK_ext_AD

    Plasmid

    #108687

    Purpose

    Plasmid for assembly of donor DNA templates consisting of three expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMTF1.1.0-gDNA

    Plasmid

    #113797

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1

    Depositor

    Kevin White

    Insert

    MTF1 (MTF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pZBTB9.1.0-gDNA

    Plasmid

    #112469

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9

    Depositor

    Kevin White

    Insert

    ZBTB9 (ZBTB9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge