Addgene

We narrowed to 876 results for: GGCT;

Showing: 781 - 800 of 876 results

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CDK6 gRNA (BRDN0001146904)

Plasmid

#75644

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK6 (CDK6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CERK gRNA (BRDN0001148273)

Plasmid

#76330

Purpose

3rd generation lentiviral gRNA plasmid targeting human CERK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CERK (CERK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

Plasmid

#171101

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Olig2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001149214)

Plasmid

#77831

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIAS-2

Plasmid

#184479

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

Plasmid

#129036

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA8 (huTSDR)

Use

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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DAPK1 gRNA (BRDN0001146680)

Plasmid

#76518

Purpose

3rd generation lentiviral gRNA plasmid targeting human DAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DAPK1 (DAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LYN gRNA (BRDN0001148376)

Plasmid

#77026

Purpose

3rd generation lentiviral gRNA plasmid targeting human LYN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LYN (LYN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRCN0000279887

Plasmid

#78153

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

hU6 and hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279953

Plasmid

#78154

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

hU6 and hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
JAK3 gRNA (BRDN0001149232)

Plasmid

#77189

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK3 (JAK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HSPB8 gRNA (BRDN0001146985)

Plasmid

#75641

Purpose

3rd generation lentiviral gRNA plasmid targeting human HSPB8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HSPB8 (HSPB8 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K7 gRNA (BRDN0001149362)

Plasmid

#77820

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-VGAT sesRNA-2a-msFlag-2a-tTA2-WPRE

Plasmid

#239030

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse VGAT (Slc32a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ClipF-HA-2a-Hu VGAT sesRNA-2a-smV5-2a-tTA2-WPRE

Plasmid

#239031

Purpose

Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Human VGAT (SLC32A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051d_sgCiPELO #1

Plasmid

#229017

Purpose

Expression of CRISPRi PELO sgRNA #1

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO (PELO Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mClover-intergenic-As

Plasmid

#209036

Purpose

Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mClover-intergenic_3-As

Plasmid

#218815

Purpose

Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETS1(12))-PGKpuro2ABFP-W

Plasmid

#208550

Purpose

Lentiviral vector expressing gRNA targeting human ETS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ETS1(12) (ETS1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CDK6-E2(12))-PGKpuro2ABFP-W

Plasmid

#200488

Purpose

Lentiviral vector expressing gRNA targeting human CDK6-E2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CDK6-E2(12) (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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