We narrowed to 10,649 results for: Uty

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pENTR1A DHFR.cHSF1

Plasmid

#134737

Purpose

Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Gateway Cloning

Tags

DHFR

Mutation

Deletion of amino acids 186-202

Promoter

None

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-soCoChR-mCardinal

Plasmid

#107713

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-mCardinal

Use

AAV

Tags

KA2 and mCardinal

Expression

Mammalian

Promoter

hSyn

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-GFP

Plasmid

#107709

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDD2270 pCS2-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

Plasmid

#251767

Purpose

TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD1707 pCS2-NES-arrb2a-(DYDIPTTENLYFQ/SGTVDAG)x1-SV40NLS-sfCherry3C(1-10)

Plasmid

#251768

Purpose

TEVp substrate (extended motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD3254 pCS2-NES-arrb2a-GGSGGSG-ENLYFQ/S-GGSGGS-SV40NLS-sfCherry3C(1-10)

Plasmid

#251769

Purpose

TEVp substrate (GS-flanked motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls

Plasmid

#251772

Purpose

Nuclear reporter for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

H2B (hist2h2l Zebrafish)

Tags

4x sfCherry3C small fragments, NLS (SV40) and NLS…

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTL51_cHS4-CAG-s36GFPHaloTag-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4

Plasmid

#223542

Purpose

PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-HaloTag, mCherry-3NLS, PAC

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pHR Myc-Oskar-FusionRed-FRB-iLID

Plasmid

#223289

Purpose

The Oskar germ granule protein tagged with optogenetic and chemogenetic tags for recruitment of desired cargo. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:

Insert

myc-Oskar-FusionRed-FRB-iLID

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

Plasmid

#233546

Purpose

A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Lentiviral

Tags

FLAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

Plasmid

#232132

Purpose

A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-tetO-mCherry-attB53

Plasmid

#183612

Purpose

RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tetO-mCherry

Use

Rmce

Promoter

tetO

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p1A CbbL-

Plasmid

#162693

Purpose

p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

CbbL K194M (inactive rubisco)

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL CD86 HLA-A201

Plasmid

#183248

Purpose

Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Inserts

CD86, 41BBL
HLA-A2 (HLA-A Human)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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