We narrowed to 1,944 results for: quo

1. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

   Plasmid

   #115698

   Purpose

   ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   SSKar2-sfpHluorin-HDEL
   

   Use

   Tags

   ER retention signal containing HDEL and ER signal…

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNC1124

   Plasmid

   #41560

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9
   

   Use

   Tags

   Expression

   Mutation

   GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

   Promoter

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS-PesaRlux

   Plasmid

   #47640

   Purpose

   wt esaR promoter in pCS26

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   PesaR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PesaR

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p006-GFP-Logic-AND

   Plasmid

   #112237

   Purpose

   GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Inserts

   GFP under control of doubly-repressible promoter
   GFP
   araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AND-gate and pLacI

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETA-qmTQ2-citrate

   Plasmid

   #225251

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-citrate
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC_mClover3_BSDminus_F0

   Plasmid

   #184165

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. hOC-GFPtpz

   Plasmid

   #110207

   Purpose

   Expresses GFP in mature bone cells.

   Depositor

   David Rowe

   Article

   Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

   Insert

   eGFPtopaz
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Human osteocalcin (BGLAP)

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.nucGAP

   Plasmid

   #78736

   Purpose

   Expression of Ca2+ sensor GAP in the nucleus

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   nucGAP
   

   Use

   Tags

   nucleoplasmin (Xenopus)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pSLHDR02_EGFP_tCD8

    Plasmid

    #124061

    Purpose

    Construction of 1st donor for scarless genome editing

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    EGFP
    tCD8
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC promoter modified

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH3

    Plasmid

    #52528

    Purpose

    serves as PCR-template to generate HR donors for C-terminal GFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eGFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-Amp-T7-roGFP

    Plasmid

    #202464

    Purpose

    Expresses redox sensitive GFP (roGFP) with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    reduction-oxidation sensitive green fluorescent protein
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMP4657

    Plasmid

    #107773

    Purpose

    Expression of EGFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EGFP in pME6010
    

    Use

    Tags

    pLacZ-EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC_mClover3_BSDminus_F2

    Plasmid

    #184167

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMP4639

    Plasmid

    #107771

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pME6010
    

    Use

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC-N-GFP

    Plasmid

    #172974

    Purpose

    MAC-tagged GFP expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    GFP
    

    Use

    Tags

    MAC-tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMP4518

    Plasmid

    #107778

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pBBR1-mcs5
    

    Use

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNC1011

    Plasmid

    #42898

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    3 tandem copies of GFP*
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    changed Phenylalanine 64 to Leucine, Serine 65 t…

    Promoter

    None

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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