167,601 results

1. pAAV_Ef1a_DIO_hTyrNull_HATag

   Plasmid

   #205285

   Purpose

   Truncated version of human Tyrosinase as control virus for Tyrosinase expression

   Depositor

   Matthias Prigge

   Insert

   hTyrosinase (truncated)
   

   Use

   AAV

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pminiTol2-4kb wnt3 promoter-Wnt3EGFP

   Plasmid

   #203793

   Purpose

   Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

   Depositor

   Thorsten Wohland

   Article

   Teh et al Development. 2015 Nov 1;142(21):3721-33.

   Insert

   Wnt3 (wnt3 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mEGFP

   Promoter

   4kb Wnt3 promoter

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1193

   Plasmid

   #143479

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF574 (ZNF574 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Vmn2r1-5Hprt-loxP TV

   Plasmid

   #108079

   Purpose

   Tergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)

   Depositor

   Peter Mombaerts

   Article

   Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

   Insert

   Vmn2r1-Hprt (5')-loxP-neo (Vmn2r1 Mouse)
   

   Use

   Mouse Targeting

   Available Since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRF1-GW YPET-T2A-mTurquoise2

   Plasmid

   #118450

   Purpose

   Produces equimolar expression of YPET and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   YPET-T2A-mTurquoise2
   

   Expression

   Yeast

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEThT-MAHS delta MTP

   Plasmid

   #90032

   Purpose

   To express His-tagged MAHS in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   MAHS
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deletion of mitochondria targeting peptide

   Promoter

   T7

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-mCherry-RORbshRNA1

   Plasmid

   #73983

   Purpose

   RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse RORb gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PG1424PGA

   Plasmid

   #74653

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PG1424PGA
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3-hPXR-FL-K277Q

   Plasmid

   #41618

   Depositor

   Sridhar Mani

   Insert

   hPXR-FL-K277Q (NR1I2 Human)
   

   Expression

   Mammalian

   Mutation

   N10D and K277Q

   Promoter

   CMV

   Available Since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

10. hSTARR-seq_ORI vector

    Plasmid

    #99296

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV9)

    Viral Prep

    #87306-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pBMN HAYFP-LOV24

    Plasmid

    #49570

    Purpose

    regulate protein stability with light

    Depositor

    Thomas Wandless

    Article

    Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

    Insert

    YFP-LOV24
    

    Use

    Retroviral

    Tags

    HA and YFP

    Mutation

    delete the last 3 aa from AsLOV2, with I532A muta…

    Promoter

    LTR

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJS002

    Plasmid

    #188325

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::GFP::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. sg_Trp53_i3-ipUSEPR-TR657

    Plasmid

    #228929

    Purpose

    Knockdown of Trp53 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Trp53_i3 (Trp53 Mouse)
    

    Use

    Lentiviral

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAK405

    Plasmid

    #37114

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2012 May;78(10):

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CamKIIa-jGCaMP8m-WPRE (AAV5)

    Viral Prep

    #176751-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CamKIIalpha

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

19. pKRaION1-N1

    Plasmid

    #177801

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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