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pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P27A)

Plasmid

#62068

Purpose

Expression of human p53 (1-73) (P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P27A

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
B8-pVTCB052

Plasmid

#231302

Purpose

Plasmid used for expression of AI-designed GFP protein B8 in E. coli

Depositor

Alexander Rives

Article

Hayes et al Science. 2025 Feb 21;387(6736):850-858.

Insert

B8

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC4 CD

Plasmid

#224251

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC7 CD

Plasmid

#224341

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC8 H143A

Plasmid

#224342

Purpose

Expresses human KDAC8 (HDAC8) H143A in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 H143A (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Histidine 143 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28:LanIV-Chondromyces

Plasmid

#225078

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Chondromyces)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanI-101

Plasmid

#225065

Purpose

Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanI-101)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2E

Plasmid

#225072

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2E)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2D

Plasmid

#225071

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2D)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

Plasmid

#166150

Purpose

Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔPrD(1-535)

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

Plasmid

#166151

Purpose

Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔIDR(1-483)

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-hMICU1EF

Plasmid

#199180

Purpose

This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

Depositor

Ming-Feng Tsai

Article

Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

MICU1 (MICU1 Human)

Tags

His6 and MBP, Thrombin site, TEV site

Mutation

D421A, E432K, F433W, F453W

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1TMSNKRS

Plasmid

#198323

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-MerTK_KRmutant-PTPN1

Plasmid

#196447

Purpose

Expression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1

Depositor

Byung Il Lee

Article

Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. d

Inserts

Tyrosine-Protein Kinase Mer (MERTK Human)
PTPN1 (PTPN1 Human)

Expression

Bacterial

Mutation

K591R, K639R, K702R, K865R

Promoter

T7 promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED(216-242)+CBPTAZ1(340-439)

Plasmid

#189961

Purpose

Coexpression of CBP TAZ1 and CITED in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-242) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

Plasmid

#189963

Purpose

Coexpression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Mutation

L243A, E245A, L246A

Promoter

t7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)

Plasmid

#189962

Purpose

Expression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-246) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

t7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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