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Showing: 8081 - 8100 of 60973 results

  1. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

    Plasmid
    #242672
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242672-plasmid-map-sequence-id-488973

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  2. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

    Plasmid
    #242674
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242674-plasmid-map-sequence-id-488969

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  3. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

    Plasmid
    #242680
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242680-plasmid-map-sequence-id-488968

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  4. pAAV-EF1a-iCreV (AAV1)

    Viral Prep
    #140135-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-EF1a-iFlpV (AAV1)

    Viral Prep
    #140137-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iFlpV (#140137). In addition to the viral particles, you will also receive purified pAAV-EF1a-iFlpV plasmid DNA. EF1a-driven, light-inducible iFlpV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-EF1a-iDreV (AAV1)

    Viral Prep
    #140136-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. CROPseq-MPSV-eBFP2

    Plasmid
    #220472
    Purpose
    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection
    Depositor
    Christian Krebs
    Article
    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    220472-plasmid-map-sequence-id-437895

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Showing: 8081 - 8100 of 60973 results