We narrowed to 12,843 results for: CHL

1. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

   Plasmid

   #20294

   Purpose

   Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Serine

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBP-lldR

   Plasmid

   #74095

   Purpose

   L-lactate repressor (LldR) from E. coli MG1655

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   lldR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LbCas12a-gRNA_BbsI_CAM

   Plasmid

   #183222

   Purpose

   LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCZGY2727

   Plasmid

   #193330

   Purpose

   Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

   Depositor

   Yishi Jin

   Article

   Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

   Insert

   Hygromycin resistance
   

   Expression

   Worm

   Promoter

   Prps-0

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJAT75

   Plasmid

   #204306

   Purpose

   Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Promoter

   ie1

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSJ111

   Plasmid

   #78707

   Purpose

   Modular vector for constitutive secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Expression

   Bacterial

   Promoter

   Pveg

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQCXIH CMV/TO DEST (w382-1)

   Plasmid

   #17394

   Purpose

   Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Retroviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCN38-yfp

   Plasmid

   #227637

   Purpose

   Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   yfp
   

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MTK2_005

    Plasmid

    #123700

    Purpose

    Encodes pCAG as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCAG
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-sgRNA-Lib

    Plasmid

    #53121

    Purpose

    sgRNA expression construction in a 3rd generation lentiviral backbone

    Depositor

    Wensheng Wei

    Article

    Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTH1

    Plasmid

    #84451

    Purpose

    shuttle plasmid for sarA P1-dsRED expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    DsRed.T3(DNT)
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Expression

    Bacterial

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNO183

    Plasmid

    #101068

    Purpose

    ppsR2-bphP1 bicistronic operon plasmid

    Depositor

    Jeffrey Tabor

    Article

    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

    Inserts

    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    insert guanine at position 430 to fix frameshift …

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCS108

    Plasmid

    #209329

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAT62

    Plasmid

    #204301

    Purpose

    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH773_pTech_PlldR-HHRz-supP(opal)

    Plasmid

    #163732

    Purpose

    Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    hhrz-supP
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC90-pmax-DEST

    Plasmid

    #48222

    Purpose

    Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR; Gateway destination

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03

    Plasmid

    #67137

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and His

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge