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169,450 results

Showing: 8121 - 8140 of 169450 results

  1. pcDNA3.1-mPCSK3 S368A

    Plasmid
    #122680
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    changed Serine 368 to Alanine
    Available Since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122680-plasmid-map-sequence-id-244743

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  4. AdenoBuilder toolkit

    Plasmid Kit
    #1000000176
    Purpose
    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.
    Depositor
    Fred Bunz
    Available Since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid
    #135391
    Purpose
    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
    Depositor
    Fan Wang
    Insert
    TeLC-P2A-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    135391-plasmid-map-sequence-id-266854

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  9. pYPQ-Cas9-Act3.0

    Plasmid
    #178954
    Purpose
    It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178954-plasmid-map-sequence-id-354300

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  11. TFORF0797

    Plasmid
    #142195
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF1 (ATF1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142195-plasmid-map-sequence-id-397672

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  12. Cilantro 2

    Plasmid
    #74450
    Purpose
    Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Available Since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74450-plasmid-map-sequence-id-140420

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  16. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid
    #221357
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221357-plasmid-map-sequence-id-436816

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  17. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid
    #221358
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221358-plasmid-map-sequence-id-436819

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  19. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid
    #118412
    Purpose
    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.
    Depositor
    Nicole Paulk
    Article
    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1
    Insert
    Firefly luciferase
    Use
    AAV, Luciferase, and Synthetic Biology
    Expression
    Insect and Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118412-plasmid-map-sequence-id-231800

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  20. pCMV-lyso-pHoenix

    Plasmid
    #70112
    Purpose
    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    lyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
    Tags
    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    70112-plasmid-map-sequence-id-418643

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Showing: 8121 - 8140 of 169450 results