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JL002: pMVP/eGFP-DEST

Plasmid

#121852

Purpose

pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-218-5p

Plasmid

#103357

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Publication

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-218-5p target

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

Plasmid

#107317

Purpose

Expresses SaCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaCas9

Use

CRISPR

Tags

3xHA and 2xNLS and NLS

Expression

Mammalian

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)

Plasmid

#104117

Purpose

synOptopatch construct

Depositor

Adam Cohen

Publication

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-dark Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

Plasmid

#85749

Purpose

Expresses anti-CRISPR protein from Type I-E and mTagBFP2

Depositor

Erik Sontheimer

Publication

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Type I-E anti-CRISPR
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LSSmKate2-Actin-C-18

Plasmid

#56209

Purpose

Localization: Actin, Excitation: 460, Emission: 605

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actin (ACTB Human)

Tags

LSSmKate2

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
HcRed1-Mito-7

Plasmid

#55842

Purpose

Localization: Mitochondria, Excitation: 588, Emission: 618

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mito (COX8A Human)

Tags

HcRed1

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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mRuby-CD151-7

Plasmid

#55850

Purpose

Localization: Tetraspanin/Membrane, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD151 (CD151 Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mRuby-Cx32-7

Plasmid

#55855

Purpose

Localization: Gap Junctions, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cx32 (GJB1 Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3.2-Caveolin-C-10

Plasmid

#57447

Purpose

Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caveolin (CAV1 Human)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos3.2-MCPA2-C-18

Plasmid

#57469

Purpose

Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCPA2 (Capza2 Mouse)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PA-mCherry-Alpha-5-Integrin-12

Plasmid

#62790

Purpose

Localization: Focal Adhesions, Excitation:400 / 504, Emission:515 / 517

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Alpha5Integrin (ITGAV Human)

Tags

mCherry

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry1-Hc-SRC-N-18

Plasmid

#57182

Purpose

Localization: Membrane/Vesicles, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hc-SRC (SRC Human)

Tags

PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only
mWasabi-Vinculin-23

Plasmid

#56512

Purpose

Localization: Focal Adhesions, Excitation: 493, Emission: 509

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vinculin (VCL Human)

Tags

mWasabi

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only
mCerulean-Farnesyl-5

Plasmid

#55367

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

mCerulean

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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mTurquoise-Actin-C-18

Plasmid

#55520

Purpose

Localization: Actin Filaments, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Actin (ACTB Human)

Tags

mTurquoise

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
mTurquoise-Caveolin-C-10

Plasmid

#55540

Purpose

Localization: Membrane, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caveolin (CAV1 Human)

Tags

mTurquoise

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SLC19A1_pCSdest

Plasmid

#53948

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SLC19A1 (SLC19A1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits