We narrowed to 19,562 results for: tec

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pcDNA3.1-HaloAKAR2.1-T2A-EGFP

Plasmid

#216489

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

Plasmid

#160736

Purpose

Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti NL

Plasmid

#113450

Purpose

Lentiviral NanoLuc control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

NanoLuc

Use

Lentiviral and Luciferase

Tags

cmyc

Expression

Mammalian

Promoter

hUbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pME464

Plasmid

#238068

Purpose

Expressed ME-ABE ABE7.10-HRHSK with SpRYCas9

Depositor

Alexis Komor

Article

Evanoff et al Nat Biotechnol. 2026 Mar 18. doi: 10.103

Insert

TadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include L36H, L51R, Y123H, C14…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Gluc-RMA-IRES-EGFP

Plasmid

#189629

Purpose

Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Promoter

IRES and hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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F-MED28

Plasmid

#15368

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED28 (MED28 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
SpCas9 TadCBEa-V106W

Plasmid

#193838

Purpose

Express TadCBEa-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa V106W-SpCas9 D10A

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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F-MED26

Plasmid

#15367

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Insert

MED26 (MED26 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-avelHCknob6xHis

Plasmid

#237383

Purpose

pTwist CMV BetaGlobin encoding avelumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

avelumab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-avelLC

Plasmid

#237384

Purpose

pTwist CMV BetaGlobin encoding avelumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

avelumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pv9_CBX7_2xChromo-13XL-BASU

Plasmid

#179413

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7.2xChromo-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149198)

Plasmid

#80248

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-BE4max

Plasmid

#183679

Purpose

Expresses eNme2-C-BE4 in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

rAPOBEC1-neNme2-C-2xUGI

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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B*27:02:01

Plasmid

#203269

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*27:02:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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A*32:01:01

Plasmid

#203287

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*32:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*13:01:01

Plasmid

#203243

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*13:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-SpRY-NLS-6xHis (gbr2101)

Plasmid

#181743

Purpose

bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

bacterial codon optimized SpRY

Use

In vitro transcription; t7 promoter

Tags

BPNLS(SV40)-6xHis and MKIEE MBP tag

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

T7

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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