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pBN237

Plasmid

#86719

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pnhr-82::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGC245

Plasmid

#19638

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R4-R3)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJM614-TRA-2::GFP

Plasmid

#27128

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 GFP (tra-2 Nematode)

Tags

GFP

Expression

Worm

Mutation

replaced stop codon with AgeI and EcoRI added GFP…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pFM111

Plasmid

#40157

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1N:FLAG (par-1 Nematode)

Tags

Flag and GST

Expression

Bacterial

Mutation

Contains aa 1-964

Promoter

tac

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pMALac-OMA-1(1-182)

Plasmid

#80350

Purpose

Expresses OMA-1 (1-182) in bacteria for protein purification purposes

Depositor

Sean Ryder

Article

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

OMA-1 (oma-1 Nematode)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Encodes AA residues 1-182

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCCM937

Plasmid

#58982

Purpose

Expresses avr-15 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-15 sgRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCCM936

Plasmid

#58981

Purpose

Expresses avr-14 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-14 sgRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pGC97

Plasmid

#19629

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGC238

Plasmid

#19631

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Tags

4xNLS

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pBN279

Plasmid

#86725

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phlh-8::FLP D5

Expression

Worm

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1702

Plasmid

#78195

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

lag-2

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pJL1603

Plasmid

#78193

Purpose

Ce cha-1 expression in SMB, SIA neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

odr-2

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1602

Plasmid

#78192

Purpose

Ce cha-1 expression in SIA, RMD neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

trp-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1601

Plasmid

#78191

Purpose

Ce cha-1 expression in IL2, SIA neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

osm-6

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pEL481

Plasmid

#52351

Purpose

GFP reporter for rack-1

Depositor

Erik Lundquist

Article

Demarco et al PLoS Genet. 2010 Nov 18;6(11):e1001215.

Insert

rack-1 (rack-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFM114

Plasmid

#40160

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N+S251A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa 1-364); S251A

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM116

Plasmid

#40162

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C+S950A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa840-1268); S950A

Promoter

tac

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pGC183

Plasmid

#19630

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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p031 pCS2 End-1 ORF

Plasmid

#11030

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End-1 (end-1 Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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