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Plasmid#114195PurposeLentivirus compatible dCas9-VPR construct driven by the EF1a promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-EGFP
Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoExpressionMutationPromoterEf1aAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianMutationPromoterHuman cytomegalovirus immediate early enhancer/pr…Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC150-PBLHL-4xHS-EF1a-DEST
Plasmid#48234PurposePiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection markerDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ngn3ER-PuroR-mCherry
Plasmid#197385PurposeTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)DepositorInsertNgn3ER-2A-PuroR-2A-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-OB-Linker-eGFP
Plasmid#176068PurposeEGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassetteDepositorInsertOB
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB-TRE-CRISPRoff-EF1A-TetOn
Plasmid#203355PurposeDrug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backboneDepositorInsertCRISPRoff-TetOn
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-EF1a-CreERT2-3Xflag-T2A-eBFP2
Plasmid#170186PurposeThis Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracingDepositorInsertCreERT2-T2A-eBFP2
UseLentiviralTagsExpressionMutationPromoterEF1 alphaAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
Plasmid#50460PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mRuby3-2A-Cre
Plasmid#203844PurposeFlp-dependent Cre and mRuby3 expressionDepositorInsertCre
UseAAVTagsmRuby3-2AExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mCherry-EGFP-LC3B
Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-LARGE1-EF1a-BSD
Plasmid#205151PurposeExpress UbC-driven LARGE1 and EF-1a driven BSDDepositorInsertLARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTS1-IRES-Puromycin
Plasmid#134665PurposeExpresses EGFP-tagged peroxisome markerDepositorInserttripeptide SKL (Peroxisome targeting signal 1)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only