We narrowed to 20,033 results for: Nov
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Plasmid#157714Purposemammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
ExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt
Plasmid#157693Purposemammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
TagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt
Plasmid#157692Purposemammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
TagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Sox17(2A-phiC31o)
Plasmid#140762PurposeTargeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.DepositorInsertphiC31o
UseMouse TargetingPromoterEndogenous Sox17Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
T/brachyury(2A-Dre)
Plasmid#140761PurposeTargeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.DepositorInsertDre
UseMouse TargetingPromoterEndogenous T/brachyuryAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRQR variant
Plasmid#138562PurposeExpresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSDDepositorInsertVRQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPIC_P2A_Hygro_Barcode
Plasmid#120484PurposeBarcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_POU1F1_P2A_Hygro_Barcode
Plasmid#120473PurposeBarcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_HOXB6_P2A_Hygro_Barcode
Plasmid#120454PurposeBarcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP306-pAAV-EFS-MCS3-pA
Plasmid#113682PurposeEFS driven Multi Cloning Site-3.DepositorInsertN/A
UseAAVPromoterCytomegalo Virus(CMV)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAEL007584-Cas9
Plasmid#100706PurposeThe promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007584-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL007584 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO NET1A-V5 WT
Plasmid#69828PurposeExpresses NET1A-V5 in mammalian cells in a doxycycline inducible mannerDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseLentiviralTagsv5ExpressionMammalianPromoterminimal CMV promoter with tetracycline operatorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx50-Hyg
Plasmid#210213PurposeIntegrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…Available sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEXP(V5-APEX2-NES)
Plasmid#107596PurposeExpresses V5-APEX2-NES in mammalian cellsDepositorInsertAPEX2-NES
UseFrt site to insert into hek293 t-rex genomeTagsNES and V5ExpressionMammalianPromoterCMV Dox inducibleAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOP-H2B-mCherry-DD
Plasmid#96894PurposeReporter to monitor translation of 5'TOP mRNADepositorInsertTOP-H2B-mCherry-DD
UseLentiviralExpressionMammalianAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only