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AB.pCCL.sin.cPPT.U6.miR-29b-Decoy.hPGK.GFP.WPRE

Plasmid

#46605

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-29b Decoy (MIR29B1, Mir29b-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pK1E-RNAP

Plasmid

#48144

Purpose

Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

rna polymerase K1E

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-500 to -1)

Plasmid

#66130

Purpose

Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)

Use

Luciferase

Promoter

VEGF -500 to -1

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-Ypet-PBD

Plasmid

#24533

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Use

Retroviral

Tags

Ypet

Expression

Mammalian

Available Since

Aug. 19, 2010

Availability

Academic Institutions and Nonprofits only
Circular 100,50 (RAB7A)

Plasmid

#170117

Purpose

AAV vector carrying a guide RNA targeting the human RAB7A mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 100,50 guide RNA

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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VEGF binding scFv G6

Plasmid

#111716

Purpose

Yeast Surface Display of G6 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6

Tags

cmyc tag

Expression

Yeast

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK/CMV)-GtwyA

Plasmid

#208574

Purpose

LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

mnucTomato
HSVtk
Neomycin
d2nucEGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

PGK/CMV and minP

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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mAMCase_CatD_6xHis_pTwistCMV

Plasmid

#200228

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

Depositor

James S Fraser

Article

Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

Plasmid

#46594

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-17 Decoy (MIR17, Mir17 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-4mtD3cpv

Plasmid

#181874

Purpose

Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

4mtD3cpv

Use

AAV and Adenoviral

Expression

Mammalian

Mutation

the 4mtD3cpv CDS differs from the recorded GeneBa…

Promoter

CaMKIIa

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pVETL-Cmcs-Flag-Ataxin3Q22-C14A

Plasmid

#22119

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pJT130_GalL_nCDA1-NGBE3

Plasmid

#145094

Purpose

Expressing base editor nCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mCyRFP2-CREB

Plasmid

#137003

Purpose

mCyRFP2-CREB donor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Tags

mCyRFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2020

Availability

Academic Institutions and Nonprofits only

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FCK-ArchT-GFP

Plasmid

#31177

Purpose

Lentiviral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pHis6-hDF-deltaTM-pCMV-9

Plasmid

#72587

Purpose

human dysferlin no transmembrane domain tagged with his tag

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

His

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
Circular 200,100 with interspersed loops (SMAD4)

Plasmid

#180191

Purpose

AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

Plasmid

#47542

Purpose

engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

Depositor

Henry Lester

Article

Frazier et al J Biol Chem. 2013 May 29.

Insert

Glutamate-gated chloride channels

Expression

Mammalian

Mutation

retention motif in ER (starting at R340) changed …

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only