We narrowed to 27,189 results for: IRE;

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pcDNA5FRT/TO-UBXD7-Strep-HA

Plasmid

#113479

Purpose

Expression of human UBXD7 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD7 (UBXN7 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

A834C silent mutation, wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Tags

T7-tag

Expression

Mammalian

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pSG5-His-SUMO1

Plasmid

#17271

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

SUMO1 (SUMO1 Human)

Tags

6xHis

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
Nurr1

Plasmid

#35000

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/myc-His-A-YAP2-deltaC

Plasmid

#21125

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Expression

Mammalian

Mutation

5 Carboxy-terminal amino acids -FLTWL are deleted

Available Since

July 2, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-SVIP-Strep-HA

Plasmid

#113491

Purpose

Expression of human SVIP with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

SVIP (SVIP Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_SIX1_P2A_Hygro_Barcode

Plasmid

#120476

Purpose

Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SIX1 (SIX1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP PICH (Nigg CB62)

Plasmid

#41163

Depositor

Erich Nigg

Article

Baumann et al Cell. 2007 Jan 12;128(1):101-14.

Insert

PICH (ERCC6L Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc hSeparase

Plasmid

#59820

Purpose

Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd2

Plasmid

#86614

Purpose

Flag tagged mouse Brd2 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd2 (Brd2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 deltaN47 Beta-catenin

Plasmid

#19287

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-47

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-wt

Plasmid

#86078

Purpose

A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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Mucolipin1-pEGFP C3

Plasmid

#62960

Purpose

full length human Mucolipin-1 cloned into pEGFP C3

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Mucolipin-1 has 1 silent mutation compared to the…

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
Ch-gelsolN

Plasmid

#37262

Depositor

Ikuo Wada

Article

Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

Insert

gelsolin (GSN Human)

Tags

mCherry

Expression

Mammalian

Mutation

deleted amino acids 380-731

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-N-Myc

Plasmid

#50772

Purpose

retroviral expression of human N-Myc

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

N-myc (MYCN Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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MuHKII-pGFPN3

Plasmid

#21922

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only