Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 164,851 results for: Des

Clear keywords
Showing: 8301 - 8320 of 164851 results

  8. pb-CAGGS-dCas9-VP64-blasto

    Plasmid
    #218556
    Purpose
    Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Article
    Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS-VP64
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218556-plasmid-map-sequence-id-431934

    Enlarge

  11. YY171: pPB_NFκB::GFP-GEMINI(A)_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

    Plasmid
    #228883
    Purpose
    PiggyBac plasmid expressing the GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-
    Insert
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
    Expression
    Mammalian
    Promoter
    pNFκB; TRE
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228883-plasmid-map-sequence-id-454808

    Enlarge

  19. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

    Enlarge

  20. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-492918

    Enlarge

Showing: 8301 - 8320 of 164851 results